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High Performance Liquid Chromatography (HPLC)/ Ultra-High Performance Liquid Chromatography (UPLC)/ Size Exclusion Chromatography (SEC)/ Gel Permeation Chromatography (GPC)/ Capillary Electrophoresis (CE)*

Equipment: Thermo Fisher TSQ

Working Principle

  • Liquids (Mobile Phase) are pumped through a column to a detector
  • Depending on choice of column, different sizes, polarity, viscosity, molecular weights can be separated by retention time to the detector
  • Analysis focuses on contents of the mobile phase

Technical Information

  • Information on composition in mobile phase
  • Molecular weights of liquids
  • Size of particles in a liquid
  • Molecule size distribution
  • Molecule charge distribution
  • Quality control tool for chemical, pharmaceutical and food industries

Detection of Adjuvants in Vaccines

  • Adjuvants increase host's immune systems when the vaccines are administered
  • Different detectors can better resolve the Adjuvants in the vaccines such as charged aerosol versus UV

List of Additional Techniques and Detectors

* Liquid Chromatography (LC)/ Gel Permeation Chromatography (GC)/
(Mass Spectrometer (MS)/ Tandem Mass Spectrometer (MS2)/
Charged Aerosol Detector (CAD)/ Electrostatic Detector(ELSD)

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Last Updated: February 17, 2020
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