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Ultraviolet and Visible Spectroscopy (UV-VIS)

Equipment: Perkin Lambda 950

Working Principle

  • Sample absorbs energy from the highest occupied energy level to the lowest unoccupied energy level
  • Measurements can be made in either transmission or reflection mode

Technical Information

  • Enzyme kinetic monitoring
  • Chemical identification
  • Dissolution testing for pharmaceuticals
  • DNA and protein activity
  • Identification of organics based on absorption
  • Quality control tool for chemical, pharmaceutical and water industry

Color Changes from Impurities in Water

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